Development of analytical method for veterinary antibiotics and monitoring of residuals in agricultural environment

Abstract Veterinary antibiotics (VAs) administered to livestock are introduced into the soil through manure and compost. These can run off surface water or leach groundwater during rainfall, causing problems such as antibiotic contamination occurrence of antibiotic-resistant bacteria. In this study, an analytical method for detecting four classes VAs (penicillin, tetracyclines, macrolides, sulfonamides) in river was developed, residual agricultural environment monitored. Soil samples were extracted with McIlvain buffer solution pretreatment conducted using solid-phase extraction, followed by liquid chromatography-tandem mass spectrometry quantify target VAs. The results study showed that recovery ranged from 62 121% 40.2–149.3% soil. Among other VAs, amoxicillin spiramycin observed have low recoveries all samples. detection limit (MDL) calculated range 2.1–12.3 ng L −1 1.2–13.2 kg soil, quantification 6.6–39.2 4.0–42.0 , respectively. This optimal then applied measure concentrations water, sediment, around Muhan watershed Korea. A total seven detected, their 0.014 0.309 μg 1.45–9.04 sediment arable be used screen is expected primary data examining fate environments.